Clinical practice and procedures for postoperative care in Sweden: Results from a nationwide survey.

A nationwide survey describing Swedish post-anaesthesia care units (PACUs), n = 75 was undertaken. The patients most commonly cared for at PACUs were patients who had undergone laparoscopic surgery, 69.3%, followed by patients who had undergone minor orthopaedic surgery, 68%. At the majority of the PACUs, 86.7%, the staff cared for emergency surgery patients and 48% for day surgery patients. In 31% of the PACUs, a pain relief service was offered through a specific pain service team. During the daytime, the anaesthetist in charge most frequently worked in the operating room 42.7%, and on call in the intensive care unit, 37.3% of the time. In 88% of the PACUs, either all or most registered nurses had a specialist education at an advanced level. The most frequent ratio of registered nurses to patients was 1 to 4, 37.3%. However, Swedish PACUS are also staffed by assistant nurses and the most frequent ratio of registered nurse to assistant nurse was 1:1. Thirty-three (44%) of the PACUs had access to a physiotherapist during the daytime. Almost all PACUs (93.3%) had predetermined discharge criteria but in the majority of PACUs' high-risk patients (68%) were not followed up by an anaesthetist after discharge from the PACU.

Inotocin, a potential modulator of reproductive behaviours in a biparental beetle, Lethrus apterus.

Several members of the highly conserved oxytocin/vasopressin neuropeptide family are involved in the regulation of reproductive and affiliative behaviours in numerous vertebrate and invertebrate species. Here we investigate gene expression patterns of inotocin, the insect ortholog of this peptide family, and its receptor to decipher their possible role in the control of reproductive behaviour in a beetle, Lethrus apterus, with biparental care. In an experiment performed on individuals of a wild population, we found that inotocin is not related to the control of water balance in this species because expression patterns did not change as a response to drought exposure. The expression levels of inotocin and its receptor, however, increased over the reproductive season i.e., when behaviour shifts from pair formation to parental care, suggesting that inotocin might be involved in the regulation of parental care in this insect. No difference was, however, found between sexes; a finding which might indicate that inotocin plays a similar role in both parents.

Long-term temporal patterns in flight activities of a migrant diurnal butterfly.

Recent studies demonstrated that the Painted Lady (Vanessa cardui), a cosmopolitan diurnal butterfly performs long-range migration between subtropical Africa and north-western Europe, covered by individuals belonging to up to six generations. Here we analyze temporal patterns of complete annual migratory activity of the Painted Lady in Hungary, located in its Central European migratory route, almost completely unstudied before. To do so, we used field occurrence data collected between 2000 and 2019 and estimated temporal patterns in migratory activity by fitting kernel density functions on the daily mean number of individuals and observation frequency. The temporal distributions of kernel density estimates were analyzed as a function of time and key climatic predictors of the study area. We found that (i) the timing of spring arrivals has been advancing; (ii) the relative intensity of the first and last migratory peaks of the Painted Lady significantly increased during the past decades; and (iii) intensity of the last migratory peak is related to the mean temperature of the previous month, inferring that the migration is shifting to earlier dates and their volume of the migration has substantially intensified, evoking mutually nonexclusive, competing hypotheses. Our study indicates the strengthening migration activities of a southerly distributed, long-distance migrant diurnal butterfly, most probably linked to the northward shift of wintering areas induced by warming trends of the southern parts of Europe. However, the complexity of the likely processes leading to changing migratory strategies calls up for further research in both breeding and wintering areas.

Evaluation of potential reference genes for real-time qPCR analysis in a biparental beetle, Lethrus apterus (Coleoptera: Geotrupidae).

Hormones play an important role in the regulation of physiological, developmental and behavioural processes. Many of these mechanisms in insects, however, are still not well understood. One way to investigate hormonal regulation is to analyse gene expression patterns of hormones and their receptors by real-time quantitative polymerase chain reaction (RT-qPCR). This method, however, requires stably expressed reference genes for normalisation. In the present study, we evaluated 11 candidate housekeeping genes as reference genes in samples of Lethrus apterus, an earth-boring beetle with biparental care, collected from a natural population. For identifying the most stable genes we used the following computational methods: geNorm, NormFinder, BestKeeper, comparative delta Ct method and RefFinder. Based on our results, the two body regions sampled (head and thorax) differ in which genes are most stably expressed. We identified two candidate reference genes for each region investigated: ribosomal protein L7A and RP18 in samples extracted from the head, and ribosomal protein L7A and RP4 extracted from the muscles of the thorax. Additionally, L7A and RP18 appear to be the best reference genes for normalisation in all samples irrespective of body region. These reference genes can be used to study the hormonal regulation of reproduction and parental care in Lethrus apterus in the future.